Fig. S3.

Infections with SINV or IAVΔNS1 induce up-regulation of OAS1, OAS2, and OAS3 gene expression in A549 cells. (A) A549 cells (number of experiments, n = 3) were infected with SINV (MOI = 5). Cells were lysed at 2, 6, 12, and 24 hpi, and RNA was isolated. OAS1, OAS2, and OAS3 mRNAs were quantified by qRT-PCR and expressed as fold-increase over levels at 2 hpi. Data are expressed as mean ± SD. (B) WT or OAS3-KO A549 cells were infected with SINV (MOI = 1) or treated with 1,000 U/mL of IFN-α. Cells were lysed 24 hpi or post IFN treatment, and proteins were analyzed by Western immunoblot with antibodies against OAS1, OAS2, OAS3, and GAPDH. (C) WT A549 cells were infected by IAVΔNS1 (MOI = 1). Cells were lysed at 0, 4, 8, 12, and 24 hpi, and proteins were analyzed by Western immunoblots with antibodies against OAS1, OAS2, OAS3, and β-actin.