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Activation of RNase L by pIC or VACVΔE3L in HT10810 or HME cells requires OAS3 expression. (A and B) HT1080 WT or KO cells were transfected with 500 ng/mL (A) or HME cells were transfected with1 μg/mL (B) of pIC, and at 4 h (HT1080 cells) or 3 h (HME cells) post transfection RNA was purified from cell lysates and analyzed on a Bioanalyzer. (C) WT or KO HME cells were infected with VACVΔE3L (MOI = 10). At 18 hpi RNA was purified from cell lysates and analyzed on a Bioanalyzer.
