Fig. S5.

IFN pretreatment reduces WNV titer in WT and OAS3-KO cells to the same level, independent of RNase L activation. (A) Cells were mock-treated or treated with 100 U of IFN-α overnight. Cells were transfected with 500 ng/mL of pIC or were infected with WNV (MOI = 20). Cells were lysed 4 h post transfection or 24 hpi, and RNA integrity was assessed on a Bioanalyzer. The position of 18S and 28S rRNA are indicated. (B) WT or OAS3-KO A549 cells were treated with 100 U of IFN-α overnight and then were infected with WNV (MOI = 1). At 36 hpi supernatants were harvested and titered for infectious virus. The data are pooled from three independent experiments carried out in duplicate. ***P < 0.001.