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. 2016 Feb 8;113(8):2241–2246. doi: 10.1073/pnas.1519657113

Table S3.

OAS1-, OAS2-, OAS3-, and RNase L-KO cells generated by CRISPR/Cas9

Parental cell line	Gene	sgRNA	Delivery method^*	Knockout cell line^†	Genotyping^‡
A549	OAS1	sgO1-2	Electroporation	A549-O1-2 C1	1-nt insertion
	OAS1	sgO1-9	Transfection	A549-O1-9 C1	1-nt insertion
	OAS2	sgO2-5	Electroporation	A549-O2-5 C2	ND
	OAS2	sgO2-9	Transfection	A549-O2-9 C1	1-nt insertion
	OAS3	sgO3-1	Transfection	A549-O3-1 C1	1-nt insertion
	OAS3	sgO3-9	Transfection	A549-O3-9 C1	1-nt insertion
	RNASEL	sgRL-6	Electroporation	A549-RL-6 C1	1-nt insertion
	RNASEL	sgRL-6	Transduction	A549-leRL-6 C1	1-nt insertion
HT1080	OAS1	sgO1-9	Transfection	HT-O1-9 C1	ND
	OAS2	sgO2-9	Transfection	HT-O2-9 C2	7-nt insertion
	OAS3	sgO3-1	Transfection	HT-O3-1 C3	1-nt insertion
	RNASEL	sgRL-6	Transfection	HT-RL-6 C1	1-nt deletion
HME	OAS1	sgO1-2	Transduction	HME-O1-2	ND
	OAS2	sgO2-9	Transduction	HME-O2-9	ND
	OAS3	sgO3-1	Transduction	HME-O3-1	ND

^*

DNA with sgRNA constructs were delivered by transfection or electroporation or by Lenti-CRIPSR transduction.

^{^†}

All knockouts were confirmed by Western blotting.

^{^‡}

The DNA fragments were amplified and sequenced and compared with the reference sequences of the genes; ND, not determined.