Table S1.
Details of base-substitution mutations
| Host | Wild type | ung | ung | ung | ||||
| Plasmid | None | None | pA3G-CTD | pA3G-CTDmut | ||||
| Independent lines | 61 | 50 | 48 | 46 | ||||
| Generation per line | 4,230 | 3,372 | 1,137 | 1,139 | ||||
| Mutations (n, %) | Mutations per nt per generation (×1010) | Mutations per nt per generation (×1010) | Mutations per nt per generation (×1010) | Mutations per nt per generation (×1010) | ||||
| Total | 246 (100) | 2.1 | 358 (100) | 4.6 | 411 (100) | 16.2 | 171 (100) | 7.0 |
| Transition | 136 (55) | 1.1 | 280 (78) | 3.6 | 368 (90) | 14.5 | 127 (74) | 5.2 |
| TA > CG | 50 (20) | 0.9 | 33 (9) | 0.9 | 19 (5) | 1.5 | 23 (13) | 1.9 |
| CG > TA | 86 (35) | 1.4 | 247 (69) | 6.2 | 349 (85) | 27.1 | 104 (61) | 8.4 |
| Transversion | 110 (45) | 0.9 | 78 (22) | 1.0 | 43 (10) | 1.7 | 44 (26) | 1.8 |
| AT > TA | 19 (8) | 0.3 | 19 (5) | 0.5 | 12 (3) | 1.0 | 9 (5) | 0.8 |
| GC > TA | 31 (13) | 0.5 | 26 (7) | 0.7 | 10 (2) | 0.8 | 9 (5) | 0.7 |
| AT > CG | 43 (17) | 0.7 | 27 (8) | 0.7 | 18 (4) | 1.4 | 16 (9) | 1.3 |
| GC > CG | 17 (7) | 0.3 | 6 (2) | 0.2 | 3 (1) | 0.2 | 10 (6) | 0.8 |
| Mutations in coding DNA | 189 (77) | 1.9 | 268 (75) | 4.0 | 315 (77) | 14.6 | 125 (73) | 6.0 |
| Mutations in noncoding DNA | 57 (23) | 3.2 | 90 (25) | 7.8 | 96 (23) | 25.6 | 46 (27) | 12.8 |
nt, nucleotides.