Table 2.

Replication strand bias in C:G to T:A mutations

Replichore	C in the LGST scored mutation		C in the LDST scored mutation		Total	Ratio of C in LGST/LDST	P value*	Ratio normalized for C†
WT
Right	C to T	30	G to A	14	44	2.1	0.06	2.3
Left	G to A	31	C to T	11	42	2.8	0.02	3.0
Total	C:G to T:A	61	C:G to T:A	25	86	2.4	0.003	2.6
ung
Right	C to T	64	G to A	55	119	1.2	0.41	1.2
Left	G to A	77	C to T	51	128	1.5	0.06	1.6
Total	C:G to T:A	141	C:G to T:A	106	247	1.3	0.05	1.4
ung (pA3G-CTD)
Right	C to T	151	G to A	35	186	4.3	4x10−11	4.6
Left	G to A	126	C to T	37	163	3.4	6x10−08	3.6
Total	C:G to T:A	277	C:G to T:A	72	349	3.8	2x10−17	4.1
ung (pA3G-CTDmut)
Right	C to T	37	G to A	17	54	2.2	0.03	2.3
Left	G to A	26	C to T	24	50	1.1	0.72	1.2
Total	C:G to T:A	63	C:G to T:A	41	104	1.5	0.08	1.6

^*P value is based on χ² test of observed versus expected values. Expected values were calculated from the ratio of cytosines in LGST/LDST in each replichore.

^†Normalized for the number of cytosines in the LGST versus the LDST (ratio = 0.941 for the right replichore and 0.936 for the left replichore).