. 2016 Feb 12;113(8):2258–2263. doi: 10.1073/pnas.1525564113

Table S1.

Frequency estimation of TSS-associated DSBs and translocations in NSPCs

NSPC genotype	Bait DSB	Input, μg	Total unique junctions	Junctions within 2 kb of the active TSS on the break-site chromosome	Translocation, % of cells	DSBs per cell
ATM^−/−	c-Myc	160	16,476	113 (Chr15)	1.37	0.55
ATM^−/−	25xI-SceI	160	16,476	113 (Chr15)	1.37	0.55
Xrcc4^−/−p53^−/−	Chr12-sgRNA-1	160	32,144	71 (Chr12)	0.44	0.18
Xrcc4^−/−p53^−/−	Chr15-Myc-sgRNA	160	21,930	45 (Chr15)	0.41	0.16

The translocation frequency, indicated as the percentage of cells with HTGTS junctions located within 2 kb of TSSs on the break-site chromosome (Chr12 or Chr15), was determined, and the rate of DSBs per cell was calculated by multiplying the translocation frequency by the total number of chromosomes (n = 40). See main text and ref. 4 for details.