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. 2016 Feb 8;113(8):2122–2127. doi: 10.1073/pnas.1520301113

Fig. S5.

Fig. S5.

Analysis of colocalization of transiently expressed HRas with EGF-Rh. (A and B) Parental HeLa cells transiently expressing YFP-HRas were incubated with 4 ng/mL EGF-Rh for 15 min at 37 °C. Single x–y confocal sections from the image z stack acquired from cells expressing low (0.5–1.0× mV-HRas expression level; “a” in A), intermediate (5.3× mV-HRas expression; “b” in A), and high levels (38.2× mV-HRas expression; B) are presented. Different confocal sections are presented in A for better visualization of endosomes in cells expressing low and intermediate levels of YFP-HRas. Arrows indicate examples of colocalization of EGF-Rh and YFP-HRas in endosomes. (Scale bar: 10 μm.) (C) HeLa/mV-HRas cells transiently expressing intermediate level of CFP-HRas (4.3× mV-HRas expression level) were incubated with 4 ng/mL EGF-Rh for 15 min at 37 °C. Single x–y confocal section from z stack of images of mVenus, CFP, and rhodamine acquired at room temperature is presented. Arrows indicate examples of colocalization of EGF-Rh and CFP-HRas in endosomes. (Scale bar: 10 μm.)