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. 2016 Feb 8;113(8):2122–2127. doi: 10.1073/pnas.1520301113

Fig. S7.

Fig. S7.

Analysis of colocalization of transiently expressed KRas with EGF-Rh in HeLa cells. (A and B) Parental HeLa cells transiently expressing YFP-KRas were incubated with 4 ng/mL EGF-Rh for 15 min at 37 °C. Single x–y confocal sections from the image z stack acquired from cells expressing low (2× mV-HRas expression; Top; A) or high (19× mV-HRas; cells are indicated by arrowheads in B) levels are presented. Arrows indicate a rare example of colocalization of EGF-Rh and YFP-KRas in endosomes. (Scale bars: 10 μm.) (C and D) HeLa/mV-HRas cells transiently expressing CFP-KRas were incubated with 4 ng/mL EGF-Rh for 15 min at 37 °C. Single x–y confocal sections from the image z stack acquired from cells expressing moderate (4.5× mV-HRas expression; C) or high levels of CFP-KRas (15× mV-HRas expression; D) are presented. Arrows indicate rare examples of colocalization of EGF-Rh and CFP-KRas in endosomes. (Scale bar: 10 μm.)