Fig. S8.

Analysis of colocalization of transiently expressed GFP-NRas with EGF-Rh in HeLa cells. Parental HeLa cells transiently expressing GFP-NRas were incubated with 4 ng/mL EGF-Rh for 15 min at 37 °C. Single x–y confocal sections from the image z stack acquired through 488-nm (GFP; green) and 561-nm (rhodamine; red) channels from cells expressing low (∼1.0× mV-HRas expression; A) or high (∼10.1× mV-HRas; B) levels are presented. Arrows indicate a rare example of colocalization of EGF-Rh and GFP-NRas in endosomes. (Scale bars: 10 μm.)