Fig. 4.

Effector T cell-intrinsic S1PR1 expression is essential for T-cell egress from dLNs after infection. gzmBER^T2/ROSAEYFP or gzmBER^T2/ROSAEYFP-S1PR1^flx/flx mice were infected as before and treated with tamoxifen starting 3 d p.i. (A) Shown at day 6 p.i. is the frequency of YFP⁺ CD8 effector T cells in the blood. The dot plots represent CD8 gated cells. (B) Confocal microscopy of dLN showing the distribution and the accumulation of GzmB-YFP⁺ effector T cells (yellow) with respect to the Lyve1⁺ (blue) lymphatic vessels. (Scale bars: 100 μm.) The data are representative of two independent experiments with at least three mice per group.