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. 2016 Feb 9;113(8):E1116–E1125. doi: 10.1073/pnas.1515741113

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Fig. 7. — Subcellular distribution of REC1 in herbicide-treated and untreated seedlings. (A) Subcellular distribution of REC1 in untreated seedlings. Whole-seedling extracts (WSE), nuclear fractions (NF), and cytosolic fractions (CF) were prepared from 8-d-old seedlings that were grown on LS medium that lacked an herbicide. Fractions were analyzed by immunoblotting with anti-histone H3 (H3), anti–UDP-glucose pyrophosphorylase (UGPase), and anti-REC1 (REC1) antibodies. Each lane contains 3 μg of protein. (B) Levels of REC1 in nuclei purified from norflurazon-treated and amitrole-treated seedlings. Seedlings were grown for 8 d on LS medium that contained either norflurazon (Nfl-treated) or amitrole (Am-treated). Whole-seedling extracts and nuclear fractions were analyzed as in A. (C) Levels of REC1 in nuclei purified from methyl viologen-treated seedlings. Seedlings were grown for 8 d on LS medium (untreated) or seedlings were grown for 5 d on LS medium and then transferred to the same medium containing 1 μM methyl viologen for 3 d (MV-treated). Whole-seedling extracts and nuclear fractions were analyzed as in A. (D) Levels of REC1 in nuclei purified from rhd3-7. Plant growth and analysis of whole-seedling extracts and nuclear fractions were as in A.