Fig. 4.

Downstream transcriptional effects of the stringent response are repressed by tRNA mischarging. qRT-PCR analyses of iraP and rrnB P1 transcripts are normalized to ompA levels and are shown relative to levels in the wild-type pheT strain in high-Phe conditions. Cultures of pheA auxotrophic strains with either wild-type PheT (solid bars) or editing-deficient PheT(G318W) (checkered bars) strains grown to early log phase in Mops minimal medium containing high Phe (0.5 mM) (green bars), low Phe (10 µM) (blue bars), or low Phe and m-Tyr (0.4 mM) (red bars). Errors bars represent the SD of three independent biological replicates.