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Transcription of the pheA leader under amino acid stress is reduced in the absence of quality control by PheRS editing. β-Galactosidase activity was monitored as described in the Materials and Methods and was determined for both wild-type pheT (solid bars) and pheT (G318W) (checkered bars) strains at late log phase. Activity from cultures grown in M9 minimal medium containing 20 µM, 200 µM Phe, and 2 mM p-Tyr are shown. Error bars represent the SD from three independent experiments.
