Fig. S3.

Reduction in ppGpp synthesis in the absence of quality control by PheRS editing is dependent on RelA. (Left) Representative TLC showing undetectable in vivo ³²P-ppGpp levels in ΔrelA, pheA auxotrophic strains with either wild-type pheT or editing-deficient pheT G318W) strains grown to early log phase in dropout medium containing high Phe (0.5 mM), low Phe (10 µM), or low Phe and m-Tyr (0.4 mM). (Right) Cold standards were run in parallel and visualized with UV light.