Table 1.

Transcriptional responses to protein stress are altered in the absence of PheRS quality control

Gene	Wild-type pheT			pheT(G318W)
	HF	LF	+m-Tyr	HF	LF	+m-Tyr
rpoH	1.3 ± 0.2	1.5 ± 0.4	1.3 ± 0.4	1.5 ± 0.4	2.6 ± 0.4	7.3 ± 0.9
dnaK	1.3 ± 0.1	0.5 ± 0.2	0.6 ± 0.3	1.0 ± 0.2	0.6 ± 0.2	8.9 ± 2.3
mopA	1.3 ± 0.1	0.6 ± 0.1	0.7 ± 0.3	1.1 ± 0.3	0.6 ± 0.1	3.8 ± 0.5
rpoE	1.3 ± 0.2	1.3 ± 0.5	1.9 ± 0.6	0.9 ± 0.1	1.6 ± 0.5	0.7 ± 0.2
ptr	1.3 ± 0.4	1.9 ± 0.3	3.0 ± 0.7	0.6 ± 0.1	1.7 ± 0.5	2.7 ± 1.1
htrA	1.3 ± 0.4	1.6 ± 0.5	1.8 ± 0.9	1.2 ± 0.3	1.4 ± 0.4	32 ± 7.6

Listed are relative transcript levels for representative genes of the sigma 32 and sigma E regulons measured by qPCR and normalized to ompA levels for either wild-type pheT or editing-deficient pheT (G318W) grown to early log phase in Mops minimal medium containing high Phe (HF, 0.5 mM), low Phe (LF, 10 µM), or low Phe and m-Tyr (+m-Tyr, 0.4 mM). Results are shown as mean ± SD of three independent biological replicates.