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. 2016 Feb 8;113(8):2300–2305. doi: 10.1073/pnas.1520441113

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Fig. S1. — Generation of N1ICD OEx mice. (A) The Pgr^+/+Rosa26^N1ICD/+ (control) and OEx mice were generated by crossing Pgr^Cre/+ mice, in which Cre recombinase is driven by Pgr promoter, with Rosa26^N1ICD/N1ICD mice, in which N1ICD together with a nuclear-localized EGFP are inserted to the Rosa26 locus after an loxP-flanked transcription termination fragment (STOP). (B) Overexpressed EGFP is visible in both epithelial and stromal cells of the uterus in OEx mice but not in control mice at 3.5 dpc. (C) Fluorescent uterine horns are visible as early as postnatal day 10 in OEx mice. (D and E) Significantly higher expression of N1ICD and EGFP mRNA confirms success of the overexpression. (F) Up-regulation of Notch signaling targets hairy enhancer of split 5 (Hes5) and hairy enhancer of split-related 1 (Hey1) confirms that overexpression of N1ICD is functional. Ctrl, control. **P < 0.01.