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. 2016 Feb 8;113(8):2300–2305. doi: 10.1073/pnas.1520441113

Table S3.

List of primers

Gene Primer application Forward sequence Reverse sequence
Notch1 qPCR intracellular domain (N1ICD) 5′-GGAGGTGGATGCTGACTG-3′ 5′-TCACTGTTGCCTGTCTCAAG-3′
EGFP qPCR 5′-AAGCTGACCCTGAAGTTCATCTGC-3′ 5′-CTTGTAGTTGCCGTCGTCCTTGAA-3′
Hey1 qPCR 5′-ACTGTCTCCACCGCTGCTCT-3′ 5′-GCTTTCCCCTCCCTTGTTCT-3′
Hes5 qPCR 5′-GAGAAGATGCGTCGGGACC-3′ 5′-GGCGAAGGCTTTGCTGTG-3′
Bmp2 qPCR 5′-CGCAGCTTCCATCACGAA-3′ 5′-GCTTCCTGTATCTGTTCCCG-3′
Wnt4 qPCR 5′-AGTGCCAATACCAGTTCCG-3′ 5′-CACACTTCTCCAGTTCTCCAC-3′
Lif qPCR 5′-TTCCCATCACCCCTGTAAATG-3′ 5′-GAAACGGCTCCCCTTGAG-3′
Pgr qPCR 5′-TGAGCCTGATGGTGTTTGG-3′ 5′-ACAGCGAGTAGAATGACAGC-3′
Ihh qPCR 5′-CCCAACTACAATCCCGACATC-3′ 5′-TCACCCGCAGTTTCACAC-3′
Areg qPCR 5′-AGATACATCGAGAACCTGGAGG-3′ 5′-AGAGACAAAGATAGTGACAGCTAC-3′
Hoxa10 qPCR 5′-GAAAACAGTAAAGCTTCGCCG-3′ 5′-GAAACTCCTTCTCCAGCTCC-3′
Nr2f2 qPCR 5′-GCCATAGTCCTGTTCACCTC-3′ 5′-GCTCCTAACGTACTCTTCCAAAG-3′
Hand2 qPCR 5′-CAGATACATCGCCTACCTCATG-3′ 5′-GGCCTTTGGTTTTCTTGTCG-3′
Esr1 qPCR 5′-AACCGCCCATGATCTATTCTG-3′ 5′-AGATTCAAGTCCCCAAAGCC-3′
Muc1 qPCR 5′-TTCCAACCCAGGACACCTAC-3′ 5′-ATTACCTGCCGAAACCTCCT-3′
Muc4 qPCR 5′-AATGTTCCTGCCTATACTGCC-3′ 5′-TTGTATGGTTCCTGGGTCAC-3′
Ltf qPCR 5′-ATCTCTGTGCCCTGTGTATTG-3′ 5′-ACATTTCCTGCCTTCTCAGC-3′
C3 qPCR 5′-AGACTGCCTGACCTTCAAAG-3′ 5′-CATCCCATCGTCCTTCTCTG-3′
Sfpi1 qPCR 5′-CAACGTCCAATGCATGACTAC-3′ 5′-ATCTGTTCCAGCTCCATGTG-3′
Rpl19 (L19) qPCR 5′-TCATGGAGCACATCCACAAGCTGA-3′ 5′-CGCTTTCGTGCTTCCTTGGTCTTA-3′
Rpl19 (L19) qPCR TaqMan Probe Catalog no. 4331182
Rbpj qPCR TaqMan Probe Catalog no. 4351372
Lif Template for cRNA probe 5′-GGTCCTTAGCAGTTCAGC-3′ 5′-GGTAGGTGGTTGCGTTG-3′
Pgr ChIP: −1,092 to −993 from TSS 5′-GAAACACTCAAAGGCATTTCCCAAAC-3′ 5′-CCGTCATAAGCTGTCCACTCTCAAGC-3′
Pgr ChIP: −755 to −644 from TSS 5′-CCCATACAGGCAGCGATGTGTTAC-3′ 5′-TGTCCAGCCTTCCTCTAACACGC-3′
Pgr ChIP: −489 to −408 from TSS 5′-TTGCTGCTCCTGCAGAGAACCC-3′ 5′-GTGAGAGCCCTCAGAGGTGAAGTTG-3′
Pgr ChIP: 240–329 from TSS 5′-CAAACATAAGAAGTCCGGAGATAGCG-3′ 5′-TTCGTGTTTCTTCTTGAATGGGAGAC-3′
Pgr ChIP: 649–735 from TSS 5′-AGGCAAAGGATCCGCAGGTTC-3′ 5′-AGTCCAAGCGTGCAAGCAAGG-3′
Pgr Bisulfite sequencing PCR 5′-TTTAGGAGATAGGGGAGGAGAAA-3′ 5′-ATAACTTCTACCCCAAAAAAAACTC-3′

Hey1, hairy enhancer of split-related 1; Hes5, hairy enhancer of split 5.