Fig. S2.

The patterns of changes in blood flow and in the diameter of vessels at the outer surface of the preovulatory follicle immediately external to the theca during the preovulatory period were similar to those observed for vessels within the theca (shown in Fig. 2). Blood flow (Upper) and vessel diameter (Lower) decreased acutely within hours of follicle rupture in Amhr^+/+SmoM2 control mice but remained relatively constant in anovulatory Amhr2^cre/+SmoM2 mutant mice in which follicular vessels are deficient in VSM. Individual vessels and the thickness of the follicle wall were measured hourly in groups of anesthetized mice studied during 2-h intervals between −2 h to 13 h after injection of hCG. Data on blood flow and vessel diameter are from 5–14 vessels from five mice (mean ± SEM) for each data point. Data points without a common super- or subscript are different (P < 0.05).