Fig. S4.

Treatment with the HIF inhibitor Ech suppressed ovulation and prevented the increase in expression of Edn2 by granulosa cells of preovulatory follicles. (A) Oocytes were recovered from the ampulla of each oviduct at 16 h after hCG injection in mice that were injected with Ech at 6 or 8 h after hCG injection or with vehicle at 8 h after hCG injection (n = 4 mice). (B) Levels of Edn2 mRNA were measured before hCG injection (0 h) and at 11 h after hCG injection in mice that were treated with Ech or vehicle at 6 h after hCG injection (n = 4 mice). Data points without a common superscript are different (P < 0.05).