Table S2.
Parameter | WT | HM | |||
SL = 2.0 μm | SL = 2.4 μm | SL = 2.0 μm | SL = 2.4 μm | SL = 2.9 μm | |
Fmax, mN/mm2 | 10.3 ± 3.7 | 21.7 ± 3.8* | 6.4 ± 2.7 | 11.0 ± 4.5 | 17.8 ± 6.0* |
EC50, μM | 5.77 ± 0.96 | 3.86 ± 0.81* | 5.53 ± 0.43 | 6.68 ± 0.39 | 4.99 ± 0.45* |
nHill | 1.6 ± 0.2 | 2.2 ± 0.5 | 1.6 ± 0.1 | 1.6 ± 0.1 | 1.8 ± 0.1 |
Force was measured over a range of [Ca2+] in permeabilized myocytes prepared from WT and HM rat hearts (Fig. S2) at SL = 2.0 μm and SL = 2.4 μm in WT myocytes and at SL= −2.0 μm, SL = 2.4 μm, and SL = 2.9 μm in HM myocytes. Passive force in HM myocytes at SL = 2.9 μm was comparable to the passive force recorded in WT myocytes at SL = 2.4 μm (Fig. S2). nHill, an index of cooperativity.
P < 0.05, long vs. short SL.