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. 2016 Feb 8;113(8):2306–2311. doi: 10.1073/pnas.1516732113

Table S2.

Myocyte force-[Ca2+] relationships

Parameter WT HM
SL = 2.0 μm SL = 2.4 μm SL = 2.0 μm SL = 2.4 μm SL = 2.9 μm
Fmax, mN/mm2 10.3 ± 3.7 21.7 ± 3.8* 6.4 ± 2.7 11.0 ± 4.5 17.8 ± 6.0*
EC50, μM 5.77 ± 0.96 3.86 ± 0.81* 5.53 ± 0.43 6.68 ± 0.39 4.99 ± 0.45*
nHill 1.6 ± 0.2 2.2 ± 0.5 1.6 ± 0.1 1.6 ± 0.1 1.8 ± 0.1

Force was measured over a range of [Ca2+] in permeabilized myocytes prepared from WT and HM rat hearts (Fig. S2) at SL = 2.0 μm and SL = 2.4 μm in WT myocytes and at SL= −2.0 μm, SL = 2.4 μm, and SL = 2.9 μm in HM myocytes. Passive force in HM myocytes at SL = 2.9 μm was comparable to the passive force recorded in WT myocytes at SL = 2.4 μm (Fig. S2). nHill, an index of cooperativity.

*

P < 0.05, long vs. short SL.