Fig. 4.

Effect of varying GSH/GSSG on Ogdh function. (A) Simultaneous examination of the effect of different GSH/GSSG ratios on ${\mathrm{O}}_2^{\bullet -}$/H₂O₂ and NADH production by purified Ogdh. (B) Traces from A were utilized to calculate the rate of ${\mathrm{O}}_2^{\bullet -}$/H₂O₂ and NADH production. GSH/GSSG ratios were achieved by varying the final concentrations of GSH and GSSG in reaction mixtures. The redox potential (E_h) obtained in the reaction mixtures was calculated using the Nernst equation. (C) Effects of GSH on Ogdh are not due to glutathione-mediated depletion of CoASH. n=4, 1-way ANOVA with Tukey's post-hoc test.