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. Author manuscript; available in PMC: 2016 Mar 3.
Published in final edited form as: Crit Rev Biochem Mol Biol. 2010 Oct 7;45(6):555–566. doi: 10.3109/10409238.2010.516741

Figure 2.

Figure 2

Serotonin induces βarr2-GFP translocation to 5-HT2AR. HEK293 cells were transfected with HA-tagged mouse 5-HT2AR (HA-5-HT2AR) and GFP-tagged β-arrestin2 (βarr2-GFP). The cells were serum-starved for 2 hours and labeled with an HA-AlexaFluor 594 antibody for 15 minutes prior to live cell imaging with a confocal microscope. In the absence of agonist, βarr2-GFP is distributed throughout the cytosol. Treatment with serotonin (5-HT) (1 μM in 2 μM ascorbate) for 5 minutes leads to βarr2-GFP translocation to the plasma membrane outlining the cells as indicated by the arrows (top). Alexa-Fluor HA staining (bottom) is shown to demonstrate the location of the 5-HT2AR in the membrane.