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. Author manuscript; available in PMC: 2016 Mar 3.
Published in final edited form as: Crit Rev Biochem Mol Biol. 2010 Oct 7;45(6):555–566. doi: 10.3109/10409238.2010.516741

Figure 3.

Figure 3

Agonist-induced internalization of 5-HT2AR-YFP expressed in WT and βarr1/2-KO MEFs. WT and βarr1/2-KO MEFs were transfected with YFP-tagged 5-HT2AR (5-HT2AR-YFP) and serum-starved for 2 hours. Under these conditions, the 5-HT2AR-YFP is localized to the plasma membrane following treatment with vehicle (2 μM ascorbate) in both cell types. Treatment of WT cells with serotonin (5-HT, 1μM) or quipazine (1μM) induces receptor internalization within 30 minutes. While serotonin fails to internalize the receptor in the βarr1/2-KO MEFs (Schmid et al., 2008), quipazine leads to internalization of the 5-HT2AR in the absence of β-arrestins.