Table 2.

Primers and Annealing Temperatures Used in Polymerase Chain Reactions to Amplify Partial OPN1SW and OPN1LW Opsin Genes in Treeshrews (Genera: Dendrogale, Ptilocercus, Tupaia) and Woolly Opossum (Genus: Caluromys).

Gene	Genus	Region	Forward (5′ to 3′)	Reverse (5′ to 3′)	Tanneal°C
OPN1SW	Ptilocercus	Exon 2 and 3	GCC TAA AGG CTT CAA GCA GGG GG	TGC CAC AGG TCT GGT GAT AGG CT	64
	Dendrogale	Exon 1	GTA CCA CCT TGC CCC TGT CT	CCT TTC CCC TGC AGT ACC T	58
		Exons 2 and 3	GGT GAT AGG CTG GTC ATT GG	CCC AGC AGC TGA GAG TAG GA	60
		Exon 4	GCT CAG CAG CAG GAG TCA G	TTC ATG AAG CAG TAG ATG ATG G	58
		Exon 5	ATG AGG CGT CTT TTC CAC AC	TGG CTT TGT TAG CAG GAA GG	60
	Tupaia	Exon 1	AAG AAC ACA ATC GGC TTT GG	GTG GCG TAG TGT CCT TTG CT	58
		Exons 2 and 3	CAG CCC AGC CTA GAA GTT TG	CCT GAC CCT CTC AAG ACC AC	62
		Exon 4	TAA TGA ATA AGG CGG GGT GA	CTG ACA AGT CAC TGG CGA GA	58
		Exon 5	ATG AGG CGT CTT TTC CAC AC	TGG CTT TGT TAG CAG GAA GG	60
	Caluromys	Exons 1–3	TGT CAG GGG ATG AGG AGT TC	GGC CAC ACG AGC ACT GTA	62
OPN1LW	Dendrogale, Ptilocercus	Exon 3	CAT CAC GGG GCT CTG GTC	CTG CTC CAA CCA AAG ATG G	60
		Exon 5	AGG CTG AGA AGG AGG TGA CA	GTG GCA CTT TTG GCG AAG TA	60
	Tupaia	Exon 3	TAC CTG TCT GCT CTT CCC TGT AG	GGT CCT AAA TGA GCC ACC CTT AC	64
		Exon 5	TGC ACT GTC CCT GTC TCA CCC AG	GGC CTG CCG ATG GCC TTA CTT AC	68