Table 3. Ectopic expression of WOR1 can overcome the effects of the wor4 deletion.
| White-to-Opaque Switching | ||||
|---|---|---|---|---|
| Strain | Media Condition | Switching Frequency (%) | n | Notesa |
| Wild Type, pMET3-Blank | Repressing | <0.60 | 168 | 1 |
| Wild Type, pMET3-Blank | Inducing | <0.32 | 308 | 1 |
| Wild Type, pMET3-WOR1 | Repressing | <0.47 | 214 | 2 |
| Wild Type, pMET3-WOR1 | Inducing | 98.96 | 384 | 2 |
| wor4/wor4, pMET3-Blank | Repressing | <0.82 | 122 | |
| wor4/wor4, pMET3-Blank | Inducing | <0.31 | 327 | |
| wor4/wor4, pMET3-WOR1 | Repressing | <0.74 | 136 | |
| wor4/wor4, pMET3-WOR1 | Inducing | 100.00 | 315 | |
| wor4/wor4, pMET3-WOR1 | Repressing | <0.49 | 203 | |
| wor4/wor4, pMET3-WOR1 | Inducing | 99.54 | 434 | |
| Opaque-to-White Switching | ||||
| Strain | Media Condition | Switching Frequency (%) | n | Notesa |
| Wild Type, pMET3-Blank | Repressing | 11.95 | 626 | |
| Wild Type, pMET3-WOR1 | Repressing | 6.71 | 472 | |
| wor4/wor4, pMET3-WOR1 | Repressing | 63.13 | 623 | |
| wor4/wor4, pMET3-WOR1 | Repressing | 64.46 | 436 | |
| wor4/wor4, pMET3-WOR1 | Repressing | 76.00 | 642 | |
| wor4/wor4, pMET3-WOR1 | Repressing | 70.01 | 670 | |
Opaque-to-white switching on the pMET3-WOR1 wor4 deletion strain was performed using opaque colonies from the white-to-opaque induction assay following several days growth on repressing media to repress WOR1 expression. As explained in the text, the wor4 deletion opaque cells are less stable than normal opaque cells.
a
1, Negative control; 2, positive control.