Table 4. TF targets of miRNAs identified by degradome sequencing.
| miRNA Family | Target Gene | TF Family |
|---|---|---|
| miR156 | MsUN014236 | SBP |
| MsUN046666 | SBP | |
| MsUN050762 | SBP | |
| MsUN011072 | SBP | |
| MsUN049469 | SBP | |
| MsUN086910 | SBP | |
| miR160 | MsUN041965 | ARF |
| MsUN097717 | ARF | |
| miR164 | MsUN043080 | NAC |
| MsUN045895 | NAC | |
| miR167 | MsUN007721 | AUX/IAA |
| miR169 | MsUN004554 | CCAAT |
| MsUN014711 | CCAAT | |
| MsUN030848 | CCAAT | |
| MsUN044017 | CCAAT | |
| MsUN096784 | CCAAT | |
| MsUN102122 | CCAAT | |
| miR396 | MsUN007041 | GRF |
| MsUN088040 | GRF | |
| MsUN104762 | C3HC4 | |
| MsUN104763 | C3HC4 | |
| MsUN101411 | MADS-box | |
| miR2645 | MsUN102295 | bZIP |
| miR5205 | MsUN046011 | AP2-EREBP |
| miR5249 | MsUN104998 | bHLH |
| miR530 | MsUN047511 | NAC |
| NmiR0007 | MsUN030863 | GRAS |
| NmiR0028 | MsUN045647 | G2-like |
The assembled alfalfa transcript sequences were submitted to the iTAK software for TF identification using default parameters; 28 TFs from 13 families were identified, and the results of hypergeometric test showed that TF genes were highly enriched among these targets.