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. 2016 Mar 3;11(3):e0149868. doi: 10.1371/journal.pone.0149868

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© 2016 Sun et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Lane M, molecular mass markers; lane 1, SDS-PAGE analysis of purified recombinant NS1 protein; Lane 2, the recombinant NS1 protein was analyzed by western blotting using SPF chicken serum for the negative control. Lane 3, western blotting of the purified recombinant NS1 protein, with chicken anti-AIV antibody and horseradish peroxidase (HRP)-labeled sheep anti-chicken IgG H+L as the first and second antibody, respectively. The arrowhead indicates the position of recombinant NS1 protein (approximate molecular mass of 30 kDa).