Fig 3. Intracellular oxidative stress during the treatment with Psi or PsiC.

T. cruzi epimastigotes from a 4-day culture were incubated with 35 μM Psi or PsiC during 4, 8 or 24 h. Intracellular oxidative stress was evaluated by flow cytometry as indicated. (a) Histograms correspond to untreated cells (curve 1, control) and treated with 35 μM Psi for 4, 8 and 24 h (curves 2, 3 and 4, respectively). As positive control, parasites were treated with 0.2 mM H₂O₂ (curve 5). (b) Time course of the Gm_t/Gm_c ratio for parasites treated with Psi or PsiC. p values < 0.05 (*) and < 0.01 (**) were considered significant.