Table 5. Cosinor analysis of circadian gene expression in the liver of WT and Lxrα-/- mice.
| Gene | Group | Mean level | Amplitude | Acrophase (ZT:min) |
|---|---|---|---|---|
| 11β-HSD1 | ♂ WT | 0.83 (0.76 ; 0.91) | 0.18 (0.09 ; 0.29) | 17:27 (15:23 ; 20:01) † |
| ♂ Lxrα-/- | 0.67 (0.60 ; 0.74)† | NSR | NSR | |
| ♀ WT | 0.90 (0.83 ; 0.98) | NSR | NSR | |
| ♀ Lxrα-/- | 0.98 (0.84 ; 1.14) * | NSR | NSR | |
| Pepck | ♂ WT | 0.47 (0.36 ; 0.58) | 0.26 (0.12 ; 0.43) | 9:06 (6:21 ; 11:21) |
| ♂ Lxrα-/- | 0.24 (0.19 ; 0.30)† | 0.12 (0.04 ; 0.19) | 8:33 (6:12 ; 11:24) | |
| ♀ WT | 0.49 (0.38 ; 0.61) | 0.24 (0.10 ; 0.41) | 11:24 (8:33 ; 14:19) | |
| ♀ Lxrα-/- | 0.42 (0.34 ; 0.50)* | 0.34 (0.23 ; 0.45) | 10:25 (9:14 ; 12:01) | |
| Per1 | ♂ WT | 0.56 (0.43 ; 0.71) | 0.32 (0.15 ; 0.52) | 12:12 (9:38 ; 14:30) |
| ♂ Lxrα-/- | 0.69 (0.54 ; 0.85) | 0.58 (0.39 ; 0.79) | 13:01 (11:37 ; 14:29) | |
| ♀ WT | 0.69 (0.53 ; 0.85) | 0.45 (0.24 ; 0.68) | 13:19 (11:15 ; 15:19) | |
| ♀ Lxrα-/- | 0.64 (0.51 ; 0.76) | 0.34 (0.19 ; 0.53) | 11:22 (9:21 ; 13:21) | |
| Bmal1 | ♂ WT | 1.38 (1.05 ; 1.72) | 1.30 (0.86 ; 1.80) | 23:41 (22:20 ; 01:00) |
| ♂ Lxrα-/- | 0.94 (0.75 ; 1.15) | 0.80 (0.54 ; 1.10) | 21:17 (19:55 ; 22:42) | |
| ♀ WT | 1.02 (0.84 ; 1.21) | 0.89 (0.64 ; 1.17) | 22:47 (21:39 ; 23:56) | |
| ♀ Lxrα-/- | 1.06 (0.90 ; 1.23) | 0.95 (0.72 ; 1.21) | 22:20 (21:20 ; 23:17) | |
| Rev-erbα | ♂ WT | 0.23 (0.17 ; 0.29) | 0.29 (0.21 ; 0.37) | 6:29 (5:25 ; 7:33) |
| ♂ Lxrα-/- | 0.20 (0.10 ; 0.32) | 0.31 (0.17 ; 0.47) | 7:42 (5:45 ; 9:42) | |
| ♀ WT | 0.41 (0.24 ; 0.60) | 0.50 (0.26 ; 0 ;78) | 7:00 (4:48; 9:03) | |
| ♀ Lxrα-/- | 0.27 (0.17; 0.39) | 0.37 (0.22; 0.54) | 6:37 (4.50; 8:23) |
For each parameter measured, values are represented as median ± 95% bootstrap confidence intervals.
* indicates significant differences between females vs males
† indicates significant differences between Lxrα-/- vs WT mice.
Circadian rhythmicity was considered significant for a p-value < 0.05; NSR, not significantly rhythmic.