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. Author manuscript; available in PMC: 2016 Mar 3.
Published in final edited form as: Int J Cancer. 2009 May 1;124(9):2050–2059. doi: 10.1002/ijc.24161

Figure 1. Effect of genistein on COX-2 expression in PCa cell lines and primary prostatic epithelial cells.

Figure 1

Figure 1

Sub-confluent cultures of LNCaP and PC-3 cells were treated with 0.1% ethanol vehicle (control, Con) or 10 M genistein (G) for 24 h. Total RNA was extracted and COX-2 mRNA levels were determined by real-time RT-PCR analysis as described in Materials and Methods. COX-2 mRNA were normalized to TBP mRNA levels and the ratios are given as fold change over control set at 1 (Panel A). Values represent mean ± SE of at least three individual experiments. * p< 0.05 as compared to control. Primary cultures of prostatic epithelial cells obtained from the peripheral zone of normal prostate tissue (E-PZ) and adenocarcinoma (E-CA) were treated with either 0.01% ethanol vehicle (control, Con) or 10 μM genistein (G) for 24 h. Total RNA was extracted and the expression of COX-2 mRNA was determined. COX-2 mRNA levels were normalized to GAPDH mRNA levels and the ratios are given as fold change over control set at 1. Values represent mean ± SE for measurements conducted in triplicate. * p< 0.05, ** p< 0.01 and *** p< 0.001 as compared to control. COX-2 mRNA expression in normal prostatic epithelial cells is shown in Panel B and that of cancer-derived epithelial cells is shown in Panel C. Panel D is a representative Western blot showing COX-2 protein expression in primary normal prostatic epithelial cell cultures (E-PZ- 3-5) after 72 h of treatment with 0.01% ethanol vehicle (control, C) or genistein at 10 μM (G). The expression of β-actin was used as a control.