PrISM screening for an Actinobacteria strain grown in three different media (ISP4, SFM, and AGS) and harvested at 4 time points (24, 48, 72, and 96 h). The proteome samples are separated on an SDS-PAGE gel and stained by Coomassie. The high molecular weight regions (>150 kDa, shown within red box) are subjected to in-gel trypsin digestion