Table 1.
An example of protein identification list (partial) from Mascot search for an Actinobacteria strain using its own sequenced genome as the database
| Protein description | Protein mass (kDa) | #Matches (sig) | #Sequence (sig) | %Coverage |
|---|---|---|---|---|
| NAD-glutamate dehydrogenase | 185 | 262 68 | 48.60 | |
| GAF sensor hybrid histidine kinase | 194 | 35 | 15 | 10.33 |
| Protein of unknown function DUF3686 | 175 | 30 | 16 | 12.54 |
| Hypothetical protein Sfla_5835 | 185 | 27 | 14 | 9.96 |
| Hypothetical protein Sfla_5465 | 169 | 18 | 15 | 11.81 |
| DNA-directed RNA polymerase, beta subunit | 144 | 18 | 12 | 11.39 |
| Amino acid adenylation domain protein | 265 | 15 | 8 | 3.80 |
| Bacterioferritin | 18 | 8 | 6 | 39.62 |
| Amino acid adenylation domain protein | 340 | 8 | 5 | 1.93 |
| Amino acid adenylation domain protein | 511 | 8 | 5 | 1.60 |
Three “amino acid adenylation proteins” (i.e., NRPSs, bolded), 511 kDa, 340 kDa, and 265 kDa each, are identified, indicating these three NRPS proteins are expressed under this growth condition