Table 2.

An example of Mascot search result (partial) using NCBInr as the database for an Actinobacteria strain which has no sequenced genome available

Peptide sequence	Protein description	Accession #
DAEALVAYcDR	Nonribosomal peptide synthetase [Streptomyces kitasatoensis]	BAH68474
FVADPFGEPGER	Nonribosomal peptide synthetase [Streptomyces griseolus]	BAH68409
ADGAVEYIGR	Pyoverdine sidechain peptide synthetase I, epsilon Lys module [Pseudomonas syringae pv. tabaci ATCC 11528]	ZP_05637427
GVGPESVVGVAVPR	Nonribosomal peptide synthetase [Rhodococcus opacus B4]	YP_002779301
ADGNVDFLGR	Amino acid adenylation domain-containing protein [Opitutus terrae PB90-1]	YP_001818855
FVADPYGAPGSR	Nonribosomal peptide synthetase [Streptomyces gibsonii]	BAH68663
LGAGDDIPIGTPVAGR	Peptide synthetase 2 [Streptomyces roseosporus NRRL 11379]	AAX31558
DVVFGTTVSGR	Amino acid adenylation domain protein [Acetivibrio cellulolyticus CD2]	ZP_07326042
FTADPYGPAGSR	Putative NRPS [Streptomyces griseus subsp. griseus NBRC 13350]	YP_001824777
GAGPETLVAVALPR	Mannopeptimycin peptide synthetase MppB [Streptomyces hygroscopicus]	AAU34203
TVAALAALAR	Putative nonribosomal peptide synthetase [Nocardia farcinica IFM 10152]	YP_119006
LGAGDDIPIGSPVAGR	Amino acid adenylation domain-containing protein [Frankia sp. EAN1pec]	YP_001510190
VVAIALPR	Peptide synthetase ScpsA [Saccharothrix mutabilis subsp. capreolus]	AAM47272
VAGPALTDFLADQR	Nonribosomal peptide synthetase [Streptomyces griseolus]	BAH68411
LVLITDEAR	Nonribosomal peptide synthetase [Streptomyces griseolus]	BAH68411
DIATAYAAR	Amino acid adenylation domain-containing protein [Streptomyces roseosporus NRRL 15998]	ZP_04696845
IPLSYAQR	Nonribosomal peptide synthetase [Streptomyces fungicidicus]	ABD65957

All the peptides related to NRPS/PKS are extracted. These peptides match to NRPS proteins from different organisms, indicating the detected NRPS share a low sequence homology with any known NRPS gene clusters in the database and likely represent a new NRPS biosynthetic pathway