A-769662 treatment induces phosphorylation of AMPK and downstream ACC in isolated cardiomyocytes. (A) Immunoblots of phosphorylated (p-) AMPK and downstream ACC in isolated cardiomyocytes from WT, AMPKα2 KO and AMPK KD mice with or without compound C (10 μM), A-769662 (100 μM) or the combination of compound C and A-769662 treatment. (B) Bars represent the relative levels of phosphorylated AMPK. Phosphorylation of AMPK was normalized to the amount of total AMPK. (C) Bars represent the relative levels of phosphorylated ACC. Phosphorylation of ACC was normalized to the amount of total ACC. Values are expressed as means ± SEM, n = 4–5 per group. *p < 0.05 vs. the corresponding control group, respectively; †p < 0.05 vs. corresponding A-769662 group, ‡p < 0.05 vs. corresponding WT group, respectively.