Fig. 2.

Contractile properties of cardiomyocytes isolated from WT, AMPKα2 KO and AMPK KD mice with or without A-769662. A-769662 facilitates the contractile function of cardiomyocytes. Freshly isolated cardiomyocytes from WT, AMPKα2 KO and AMPK KD mice were incubated with or without A-769662 (100 μM) prior to mechanical assessment. (A) Resting sarcomere length; (B) peak height; (C) maximal velocity of shortening (+dL/dt); (D) maximal velocity of re-lengthening (−dL/dt); (E) peak shortening (normalized to the resting sarcomere length); (F) time-to-90% relengthening (TR90); and (G) representative sarcomeric shortening traces obtained from WT, WT + A-769662, AMPKα2 KO, AMPKα2 KO + A-769662, AMPK KD + A-769662 isolated cardiomyocytes. Means ± SEM, n = 50–120 cells from 2 to 4 mice per group,*p < 0.05 vs. vehicle group, respectively; †p < 0.05 vs. WT vehicle group.