A-769662 significantly increased cardiomyocyte contractility without changing the amplitude of Ca2+ transient. (A) Histograms showing the resting intracellular calcium level; (B) histograms showing mean Ca2+ transient amplitude; (C) the mean time constant of Ca2+ transient decay (Tau); (D) representative Ca2+ transient traces obtained from WT, WT + A-769662, AMPKα2 KO, AMPKα2 KO + A-769662, AMPK KD and AMPK KD + A-769662 isolated cardiomyocytes. Mean ± SEM, n = 50–120 cells from 2 to 4 mice per group.