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A-769662 treatment induces phosphorylation of AMPK downstream ACC and cardiomyocyte troponin I (cTnI). (A) Immunoblots of phosphorylated (p-) ACC and cTnI (Ser149) of isolated cardiomyocytes from WT, AMPKα2 KO and AMPK KD mice with or without A-769662 (100 μM) treatment. (B) Bars represent the relative levels of phosphorylated cTnI (Ser149). Phosphorylation of cTnI (Ser149) was normalized to the amount of β-actin; mean ± SEM, n = 4 per group.
