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. 2016 Mar 3;11(3):e0149754. doi: 10.1371/journal.pone.0149754

Fig 4. Atg7 knockdown extravagantly activated p38 and NF-κB signaling in Raw264.7 macrophages.

Fig 4

A. ELISA analysis of pro-inflammatory cytokines in the supernatant of cell cultures upon ConA challenge. B. Western blotting analysis of p-p38, p-IκB and nuclear p65 protein expression in cultured cells upon ConA challenge. Differences in p38 and IκB phosphorylation were quantitated using densitometry. * p<0.05 and ** p<0.01 vs. controls. C. EMSA results of NF-κB p65 activation and translocation upon ConA challenge. Raw264.7 cells were transfected with Atg7 siRNA or control siRNA and treated with 10 μg/ml ConA for 2 h. Representative results were from 3 independent experiments. Data are presented as mean±SD; * p<0.05 and ** p<0.01 vs. controls.