Fig 5. Effect of mutation of heavy chain CDR3 on D5 scFv binding to EV71 particles and SP70 peptide.

The binding of D5 scFv variants with point mutations at heavy chain CDR3 region to (A) F-particle, (B) E-particle, (C) VLP, and (D) SP70 peptide were determined by ELISA assay. The irrelevant hepatitis B core protein (HBc) was used as negative control. Mean values and standard deviations of duplicate samples are shown.