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. 2016 Mar 3;11(3):e0150477. doi: 10.1371/journal.pone.0150477

Fig 5. P-CTD mediates nuclear import of GFP dependent on IMPα2 and IMPβ1.

Fig 5

Nuclear import of purified GFP-P-CTD and GFP-T-ag NLS was analyzed as described in the legend to Fig 4 except that exogenous cytosol was pre-incubated (15 min) with the indicated antibodies. (A) CLSM images following 30 min incubation of GFP-P-CTD and GFP-T-ag NLS in the presence of the indicated antibody are shown. (B) Images such as those in A were analyzed to determine the Fn/c as described in the legend to Fig 2 (mean ± S.E.M., n ≥ 3 nuclei for each time point; data are from a single assay typical of three separate assays). Curve fitting used GraphPad Prism 6 (one-phase association).