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. 2016 Mar 3;11(3):e0150609. doi: 10.1371/journal.pone.0150609

Fig 1. The sequence alignment, phylogenetic tree analysis and hydropathy curve of AdLEA.

Fig 1

(A) Alignment of deduced amino acid sequence of AdLEA with other closely related LEA proteins from other plant species. The conserved “LEA_2” motif (PF03168) is boxed. AdLEA (Arachis diogoi, GU223575); GmLEA (Glycine max, KHN11020.1) NtLEA (Nicotiana tabacum, ABS50432.1) MtLEA (Medicago truncatula, XP_013459494) AtLEA (Arabidopsis thaliana, BT024723). (B) Hydropathy curve for AdLEA protein. (C) Phylogenetic analysis of AdLEA with LEA homologues from different plant species. The divergence of the clades between the monocots and dicots is highlighted. Bootstrap values are indicated at the branches. The accession numbers of sequences used for construction of phylogenetic tree are as follows: SiLEA14 (Setaria italica, KJ767551); AtLEA14 (Arabidopsis thaliana, NM_100029); Lea14-A (Zea mays, NM_001159174); D95-4 (Glycine max, U08108); IbLEA14 (Ipomoea batatas, GU369820); ER5 (Solanum lycopersicum, U77719); Lemmi9 (Solanum lycopersicum, Z46654); CaLEA6 (Capsicum annuum, AF168168); OsLEA5 (Oryza sativa, JF776156); pcC27-45 (Craterostigma plantagineum, M62990)); pcLEA14 (Pyrus communis, AF386513); At1g01470 (Arabidopsis thaliana, BT015111); LEA14-A (Gossypium hirsutum, M88322); Lea14 homolog (Glycine max, NM_001251780); At2g46140 (Arabidopsis thaliana, NM_130176); Os01g0225600 (Oryza sativa, NM_001048996); LEA14-A-like (Brachypodium distachyon, XM_003567779); LOC100274480 (Zea mays, NM_001148839); SORBIDRAFT 09g029860 (Sorghum bicolour, XM_002441543); LEA-like protein (Cenchrus americanus, AY823547); OsI21161 (Oryza sativa, CM000130); Os05g0526700 (Oryza sativa, NM_001062639); Os05g0584300 (Oryza sativa, NM_001062985); At2g44060 (Arabidopsis thaliana, BT024723); LOC100285131 (Zea mays, EU970969); CISIN_1g018706mg (Citrus sinensis, KDO49745); Th LEA protein (Tamarix hispida, AHF21584.1); PHAVU_008G203600g (Phaseolus vulgaris, XP_007141526); Lea14-A (Ricinus communis, XP_002533345); GmLEA (Glycine max, KHN11020.1) Mt LEA protein (Medicago truncatula, XP_013459494); chilling-responsive protein (Nicotiana tabacum, ABS50432); putative LEA-1 protein (Rosa chinensis, AKC88473).