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. 2016 Mar 3;11(3):e0150609. doi: 10.1371/journal.pone.0150609

Fig 9. Effect of oxidative stress on AdLEA transgenic plants.

Fig 9

(A and C) WT and transgenic seedlings after growth on MS medium supplemented with 5 and 10μM MV respectively. (B and D) Recovery of seedlings on MV free medium. (E) Chlorophyll content (μg mg-1 FW) in the seedlings after growth on MV supplemented medium. (F) Phenotypic differences in the leaf discs from WT and transgenic plant lines floated in dose-dependent concentrations (5 and 10μM) of MV. (G) Chlorophyll content (μg mg-1 FW) in the leaf discs after treatment with MV. (H) Lipid peroxidation expressed as TBARS content (μmol g-1 FW) in leaf discs after treatment with MV. All the experiments were performed in triplicates and data represented as mean ± SD (n = 3; biological replicates). 11 seedlings per plate constitute one biological sample for seedling assay. 15 leaf discs from a single leaf constitute one biological sample for disc senescence assay. Statistical analysis was performed with two-way ANOVA (*P<0.05, **P<0.001).