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. 2016 Mar 3;11(3):e0149633. doi: 10.1371/journal.pone.0149633

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© 2016 Chen et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) The florescent images of the HCT116 cancer cell released from the Texas Red-SLB coated chip. The cell was wrapped with Texas Red-SLB lipid molecules around the membrane (blue: DAPI; green: pre-stained CellTracker Green CMFDA; red: Texas Red conjugated lipid molecule). (B and C) Overlaid fluorescent images of the released single CTC and CTM for cell characterization. Eluted cells from clinical samples were categorized as CTC by size, morphology and immunostainning (DAPI+/CK20+/CD45-). WBCs were identified by DAPI+/CK20-/CD45+ immunostaining (blue: DAPI; green: CD45; red: CK20).