Table 1. Genetic mutation analyses of eluted CTCs.
Hotspot analyses of randomly selected 3 healthy (H1~H3) and 10 colorectal cancer patient samples (P1~P10) by castPCR. The mutation loci detected include 3 loci for p53, 2 loci for APC, and 2 loci for KRAS. (Loci with ΔCt value < 9.96 indicate positive and were represented in bold format).
| TP53R248W | TP53R175H | TP53R248Q | APC1309fs*3 | APC1556fs*3 | KRASG12V | KRASG13D | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ID | CTC/2mL | Ct | ΔCt | Ct | ΔCt | Ct | ΔCt | Ct | ΔCt | Ct | ΔCt | Ct | ΔCt | Ct | ΔCt |
| H1 | 0 | NVD | NA | NVD | NA | 35.0894 | 11.257 | NVD | NA | 33.9754 | 10.2039 | NVD | NA | NVD | NA |
| H2 | 0 | NVD | NA | 36.2758 | 12.3402 | NVD | NA | NVD | NA | 33.7421 | 10.7723 | NVD | NA | NVD | NA |
| H3 | 0 | 39.2909 | 15.4595 | 34.6825 | 10.8511 | NVD | NA | NVD | NA | 33.6487 | 10.7724 | NVD | NA | NVD | NA |
| P1 | 174 | NVD | NA | 38.8593 | 14.4028 | 33.831 | 9.3745 | NVD | NA | 37.9785 | 13.7704 | NVD | NA | NVD | NA |
| P2 | 28 | NVD | NA | NVD | NA | NVD | NA | NVD | NA | 33.1786 | 8.5154 | NVD | NA | NVD | NA |
| P3 | 26 | NVD | NA | 39.8006 | 15.3288 | NVD | NA | NVD | NA | 35.7989 | 11.1062 | NVD | NA | NVD | NA |
| P4 | 12 | NVD | NA | 39.1121 | 15.078 | 37.81 | 13.7759 | NVD | NA | 32.861 | 8.8555 | NVD | NA | NVD | NA |
| P5 | 6 | NVD | NA | 37.5928 | 13.5116 | NVD | NA | NVD | NA | 33.3171 | 9.2162 | NVD | NA | NVD | NA |
| P6 | 2 | NVD | NA | 35.608 | 10.9916 | 34.1655 | 9.5491 | NVD | NA | NVD | NA | NVD | NA | NVD | NA |
| P7 | 2 | NVD | NA | 38.4027 | 13.9292 | NVD | NA | NVD | NA | 34.4292 | 9.6906 | NVD | NA | NVD | NA |
| P8 | 6 | NVD | NA | 39.4417 | 15.4701 | NVD | NA | NVD | NA | 32.0008 | 8.5251 | NVD | NA | NVD | NA |
| P9 | 4 | NVD | NA | 38.6133 | 14.5613 | 39.4851 | 15.4331 | 37.0999 | 12.8839 | 36.1388 | 11.9228 | NVD | NA | NVD | NA |
| P10 | 4 | NVD | NA | NVD | NA | NVD | NA | NVD | NA | 34.9676 | 10.9355 | NVD | NA | NVD | NA |