Fig 5.

In the situation of castration-resistant prostate cancer cells, C4-2 (A, C, E) and PC3 (B, D, F), serum induced the expression of SLC45A3-ELK4, reduced CTCF expression and reduced CTCF binding to the insulator(s). (A) (B) SLC45A3-ELK4 expression level was measured by qRT-PCR, normalized against GAPDH, and further normalized against the level in “Serum-”. (C) (D), CTCF expression level measured by qRT-PCR, normalized against GAPDH, and further normalized against the level in “Serum-”. (E) (F), Binding of CTCF to the two insulators measured by ChIP and qPCR. IgG was used as negative control for the CTCF antibody. * p<0.05, **p<0.01.