Skip to main content
. 2016 Mar 3;12(3):e1005902. doi: 10.1371/journal.pgen.1005902

Fig 4. Activity of the Mos1 and Hsmar1 promoter in presence or absence of their Δ7 segment.

Fig 4

(a) Schematic representation of expression cassettes containing a luciferase reporter gene that were used to evaluate the impact of the DNA element under investigation (EUI; here Δ7 segment) on their promoter pMos or pHsmar1 that are composed of the 5’ inverted terminal repeat plus the 5’ untranslated terminal region (black arrows). The DNA sequences of pMos and pHsmar1 are supplied in S3 Fig. The assay is based on the transient expression of two plasmids: (i) the pRL-Tk plasmid that expresses the Renilla luciferase under control of a Thymidine kinase promoter as a control for transfection efficiency, (ii) a derivative of the pGL3 plasmid that expresses the Firefly luciferase under control of an early SV40 promoter. Effect of Δ7-MOS1 (b) and Δ7-HSMAR1 (c) DNA segments on their own promoter in HeLa cells. Results are represented by median values from three experiments done in triplicate. Bars correspond to quartiles 1 and 3. * indicates significant differences (p<0.05) between the pMos1-Luc-Δ7, pHsmar1-Luc-Δ7, and pMos1-Luc or pHsmar1-Luc, respectively.