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. 2016 Mar 3;12(3):e1005902. doi: 10.1371/journal.pgen.1005902

Fig 8. Location of regions essential to silencing activity within the Δ8 segments of MOS1.

Fig 8

(a) Variant fragments within the sequences of the Δ8-MOS1. Dashes indicate positions that are present in each fragment. (b) Expression of the Firefly and the Renilla luciferase marker genes using transient expression assays in HeLa cells. The assays were performed with Δ8-MOS1 and five variants cloned in + orientation. (c) and (d) Impact of the Δ7 segments of MOS1 on the expression of the Firefly and the Renilla luciferase marker genes using transient expression assays in cell lineages H4 (TARBP2+/NRSF-/YY1+/NFAT-5-) (c) and DT40 (TARBP2+/NRSF+/YY1+/NFAT-5-) (d). In (b), (c) and (d), each histogram bar corresponds to the median value obtained from three experiments done in triplicate. Bars correspond to quartiles 1 and 3. The median ratio RLU from Firefly/RLU from Renilla were calculated as indicated in Fig 2. The area where the ratios “RLU from Firefly/RLU from Renilla” were above 1 (i.e. where no strong silencer effect is observed) is coloured in grey. In (b) * and ** indicate a significant difference (p<0.05) with the P_Luc controls. ** also indicates a significant difference (p<0.05)with HS2_P_Luc_Δ8-MOS1.