Table 1. Viral Loads Determined by Real Time Quantitative PCR after Nasal or Intragastric Infection (expressed as genomic equivalents/mg wet weight for solid organs and stool and copies/mL for body fluids).
3dpia | 7dpi | 10dpi | 14dpi | 21dpi | 28dpi | |
---|---|---|---|---|---|---|
Kidney | ||||||
Nasal | 0 | 2.41×101±3.49×101b | 2.16×103±3.83×103 | 1.85×103±3.58×103 | 3.85×101±7.70×101 | 0 |
Intragastric | 0 | 0 | 0 | 0 | 3.64×102±7.27×102 | 0 |
Lung | ||||||
Nasal | 3.52×105±6.60×105 | 2.05×106±2.08×106 | 2.62×106±2.35×106 | 7.98×105±3.08×105 | 1.70×105±1.56×105 | 1.07×105±9.85×104 |
Intragastric | 4.56×102±3.67×102 | 6.28×101±8.52×101 | 1.46×101±2.06×101 | 1.76×101±3.52×101 | 1.45×103±1.16×103 | 5.20×100±1.04×101 |
Heart | ||||||
Nasal | 0 | 0 | 1.51×103±2.99×103 | 4.67×102±9.33×102 | 1.92×101±3.84×101 | 2.63×102±5.26×102 |
Intragastric | 0 | 0 | 0 | 0 | 0 | 0 |
Aorta | ||||||
Nasal | 8.54×104±1.49×105 | 1.98×105±2.32×105 | 6.65×104±5.29×104 | 7.09×104±1.38×105 | 8.57×102±1.71×103 | 6.09×102±1.20×103 |
Intragastric | 0 | 0 | 0 | 0 | 2.02×102±4.02×102 | 0 |
Stool | ||||||
Nasal | 1.27×101±7.78×100 | 1.19×103±1.88×103 | 7.33×101±3.22×101 | 3.50×101±1.32×101 | 3.36×100±6.71×100 | 7.83×10−1±1.56×100 |
Intragastric | 0 | 0 | 0 | 0 | 0 | 0 |
Colon | ||||||
Nasal | 0 | 6.84×102±1.36×103 | 4.74×101±9.47×101 | 1.95×101±3.90×101 | 2.61×101±4.37×101 | 3.17×101±3.70×101 |
Intragastric | 0 | 0 | 0 | 0 | 0 | 0 |
Spleen | ||||||
Nasal | 0 | 1.95×104±3.57×104 | 1.70×104±1.60×104 | 1.73×104±2.55×104 | 9.83×103±1.44×104 | 1.07×102±6.13×101 |
Intragastric | 0 | 0 | 0 | 2.12×101±4.25×101 | 7.15×102±1.41×103 | 0 |
Urine | ||||||
Nasal | 0 | 0 | 0 | 0 | 0 | 0 |
Intragastric | 4.89×102±9.79×102 | 0 | 0 | 0 | 0 | 0 |
Serum | ||||||
Nasal | 0 | 1.50×104±1.77×104 | 0 | 0 | 0 | 0 |
Intragastric | 0 | 0 | 1.50×101±1.77×101 | 0 | 0 | 0 |
a dpi, days post infection
b Values are mean +/- sd.