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. 2016 Mar 3;11(3):e0150893. doi: 10.1371/journal.pone.0150893

Fig 2. SAA induces chemokine and MMP 9 expression in HSCs in an NF-κB- and JNK-dependent manner in HSCs.

Fig 2

Activated human HSCs were treated with rhSAA in the prescence or absence of JNK inhibitor SP600125 (20 μM), or after infection with adenoviruses expressing either IκBsr or GFP. A. 24 hours after rhSAA treatment, supernatants were analyzed for MCP-1, IL-8 and RANTES secretion by ELISA. B-C. 6 hours after rhSAA treatment, RNA was extracted and analyzed for mRNA levels of MCP-1, IL-8 and RANTES (B) or MMP9 (C) by quantitiative real time PCR (*p<0.05 vs. SP600125 treatment or IκBsr infection, resp.). D. 24 hours after SAA treatment, proteins were extracted and MMP9 activity was analyzed by zymography.